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  • Title: [The effect of expressed human apolipoprotein AI on plasma high density lipoprotein level in transgenic mice].
    Author: Li H, Gu S, She M.
    Journal: Zhonghua Yi Xue Za Zhi; 1998 Jul; 78(7):531-3. PubMed ID: 10923465.
    Abstract:
    OBJECTIVE: To study the regulation of human apolipoprotein AI(h-apo AI) gene expression and its role in high density lipoprotein (HDL) metabolism. METHODS: Transgenic C57BL/6 mice established in this laboratory with human apoAI gene containing mouse metallothionein-I(MT-I) promoter were used for investigation. RESULTS: Southern blot identified the presence of 4-15 copies of h-apo AI gene in the transgenic mice. Northern blot showed that human apo AI mRNA was expressed mainly in the liver and kidneys, and the high level of h-apo AI mRNA was obtained in liver, kidneys and small intestine after Zinc(Zn) induction. Total Plasma apoAI Level in transgenic mice was significantly increased than that in the controls, and a high h-apoAI level was detected in the transgenic mice (46% increased after Zn induction). Additionally, total and HDL cholesterol levels were noticed to be highly increased to 47% before and 103% after Zn induction in the transgenic mice comparing to that of the controls. The plasma HDL and h-apoAI levels were significantly correlated (r = 0.85, P < 0.01) in the transgenic mice. CONCLUSION: Apo AI has a profound effect on regulating HDL levels in the transgenic mice. This animal model is considered appropriate for studying the effect of apoAI on lipid metabolism and the mechanism combating atherogenesis.
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