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  • Title: [Human bone morphogenetic protein-2: recombinant expression in E. coli].
    Author: Ma Q, Dang G, Ma D.
    Journal: Zhonghua Yi Xue Za Zhi; 1998 Apr; 78(4):308-10. PubMed ID: 10923494.
    Abstract:
    OBJECTIVE: To explore the way of producing human bone morphogenetic protein-2(hBMP-2) for bone healing by using the gene engeneering techniques. METHODS: hBMP-2 cDNA fragment, which consists of 3' end partial propeptide and mature peptide sequence, was inserted into the multiple cloning site of expression vector pkpL-3a via ligation. The recombinant plasmid pkpL-3a/hBMP-2 was transformed into E. coli pOp2136. By the method of restriction map, the positive expression clone was selected. SDS-PAGE analysis showed a new foreign protein band near 27,000 after induction. The yield of induced hBMP-2 accouned for 10% of the total bacterial proteins. RESULTS: The partial purified recombinant hBMP-2 was implanted into Wistar rat thigh. After 4 weeks, histological analysis showed that it induced the proliferation of mesenchymal type cells and formation of new cartilage and bone in the implantation area. CONCLUSION: The hBMP-2 produced by gene engeneering techniques has the biologic capacity of ectopic bone formation.
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