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  • Title: Replication origin of Streptococcus pyogenes, organization and cloning in heterologous systems.
    Author: Suvorov AN, Ferretti JJ.
    Journal: FEMS Microbiol Lett; 2000 Aug 15; 189(2):293-7. PubMed ID: 10930754.
    Abstract:
    The origin of DNA replication (oriC) of Streptococcus pyogenes, group A streptococci (GAS), has been cloned in Escherichia coli and reintroduced by transformation into other GAS strains. Transformation frequencies into GAS strains with oriC-carrying plasmids occurred with unusually high frequencies. However, the oriC-containing plasmids in the new recipients were found to be unstable and had a tendency to integrate into the chromosome, even when a recA GAS strain was used as a recipient. The GAS oriC was able to direct the replication of autonomous plasmids in group B streptococcal recipients. The chromosomal organization of the oriC region of GAS relative to other bacterial species appears to be similar to oriC of Bacillus subtilis and other Gram-positive microorganisms.
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