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  • Title: Accessibility of chromatin to DNA polymerase I and location of the F1 histone.
    Author: Saffhill R, Itzhaki RF.
    Journal: Nucleic Acids Res; 1975 Jan; 2(1):113-9. PubMed ID: 1093142.
    Abstract:
    The effect of prebound poly-L-lysine upon the template activity of DNA, chromatin and F1 histone-depleted chromatin for E. coli DNA polymerase I has been investigated. Measurements have been made in the absence and presence of 0.1M NaCl. From the results we conclude that only ca 60% of the polylysine-accessible DNA, i.e. 22% of the total DNA of the chromatin, is accessible to the DNA polymerase I and that ca. 30% of the polylysine-accessible DNA, i.e. 11% of the total DNA, is associated with the F1 histone.
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