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  • Title: Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.
    Author: Lewis JB, Atkins JF, Anderson CW, Baum PR, Gesteland RF.
    Journal: Proc Natl Acad Sci U S A; 1975 Apr; 72(4):1344-8. PubMed ID: 1093178.
    Abstract:
    Cytoplasmic RNA, isolated from cells late after infection by adenovirus type 2 and fractionated by hybridization to specific fragments of adenovirus DNA produced by cleavage with the endonuclease R-EcoRI, was used as template for protein synthesis in cell-free mammalian extracts. Each of the R-EcoRI fragments of DNA selects RNA that encodes specific subsets of the viral polypeptides. From the known order of the R-EcoRI fragments, the following partial map is deduced: (III, IIIa, IVa2, V, P-VII, IX), (II, P-VI), 100K, IV-where the relative order of the components enclosed in parentheses has not yet been determined.
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