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  • Title: Comparison of five methods for the detection of measles specific immunoglobulin G antibody.
    Author: Vázquez-Rosales JG, Solórzano-Santos F, Bustamante-Calvillo ME, Torres-López J, Díaz-Ponce H, Muñoz O, Alvarez-Muñoz MT.
    Journal: Rev Latinoam Microbiol; 1996; 38(3-4):167-75. PubMed ID: 10932707.
    Abstract:
    In this work we compare the sensitivity, specificity and predictive values of hemagglutination inhibition (HI), immunofluorescent assay (IFA), biotin-streptavidin immunofluorescent assay (B/SA-IFA), enzyme-linked immunosorbent assay (EIA) and plaque neutralization test (PN). This study includes serum samples from children taken before and after vaccination, children with clinically diagnosed measles and household contacts. EIA were the most specific and better serological diagnostic test. HI and IFA had high sensitivity but low specificity. An alternative to EIA is B/SAIFA, which is cheaper than EIA and can be used in the study of small outbreaks or in isolated cases.
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