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  • Title: Lectin activity from the cattle tick (Boophilus microplus) saliva.
    Author: Bautista-Garfias CR, Martínez-Cruz MA, Córdoba-Alva F.
    Journal: Rev Latinoam Microbiol; 1997; 39(1-2):83-9. PubMed ID: 10932717.
    Abstract:
    The capability of Boophilus microplus saliva (BmS) and lectins to induce immunosuppression in mice and to agglutinate erythrocytes from distinct animal species were compared. CD-1 mice were distributed into groups (two for each substance) of 4-5 animals/group and treated intraperitoneally (i.p.) with 50 mg and 150 mg of BmS; concanavalin A (Con A); potato's lectin (PL), striped bean lectin (SBL) or 0.1 ml of phosphate buffered saline (PBS). All mice were immunized i.p. with 2 x 108 sheep erythrocytes (SE) 48 hours after treatment. Five days after immunization sera was obtained from mice to determine anti-SE antibody titer by direct hemagglutination. The mean antibody titers (Log2) obtained was 5.5 and 4.2 for BmS, 4.4 and 0 for Con A, 5.2 and 4.8 for PL, 4.6 and 0 for SBL and 7.4 for PBS. BmS and lectins were tested to agglutinate mouse (M), rat (Rt), rabbit (Rb), sheep (S) and human A (HA), human B (HB) and human O (HO) erythrocytes. BmS agglutinated Rb, S, HA, HB and HO erythrocytes at titer (Log2) of 1, 1, 6, 1 and 2 respectively. Con A and PL agglutinated erythrocytes from all species tested, while SBL agglutinated M, S and HA erythrocytes. A 50% inhibition of agglutination of HA erythrocytes by BmS was observed when D(+) Galactosamine and D(+) Glucosamine, at 0.2 M, were used. The results obtained indicate that BmS contains a weak nonspecific lectin which is inhibited by aminated sugars, and that induces immunosuppression in mice as other lectins.
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