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  • Title: Sensitivity and specificity of an ELISA as a screening test for the diagnosis of Brucella ovis in sheep.
    Author: Núñez-Torres ED, Díaz-Aparicio E, Hernández-Andrade L, Trigo-Tavera FJ, Suárez-Güemes F.
    Journal: Rev Latinoam Microbiol; 1997; 39(3-4):123-8. PubMed ID: 10932721.
    Abstract:
    A hot saline extract of Brucella ovis strain REO 198 at a concentration of 5 micrograms/ml in phosphate buffer pH 7.2 was used to adsorb onto Maxisorb plates and incubated at 37 degrees C during 12 h; unadsorbed excess antigen was washed off thrice with phosphate buffer containing 0.5% Tween 20. As blocking agent 1% skim milk was used. The conjugate used was protein G bound to peroxidase diluted 1:100. Thirty three sheep sera from bacteriologically confirmed infected animals and 39 sheep sera from healthy animals from disease-free zones were used. Sera were diluted 1:200. ELISA's sensitivity was 97% and specificity 84%. The cut-off value was chosen for a high sensitivity (100%) despite some loss of specificity in order to diminish false negative results rendering thus a suitable screening test for sheep epididimitis caused by Brucella ovis.
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