These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Transport of large neutral amino acids into BeWo cells.
    Author: Eaton BM, Sooranna SR.
    Journal: Placenta; 2000; 21(5-6):558-64. PubMed ID: 10940206.
    Abstract:
    BeWo choriocarcinoma cells were cultured onto solid microcarrier beads, packed into columns and superfused. Unidirectional influx of l -phenylalanine (l -phe) and l -leucine (l -leu) across the microvillous border of the cells was studied using a rapid paired-tracer dilution technique. Influx of l -phe and l -leu comprised both saturable and non-saturable components. K(m)values for l -phe and l -leu were 0.57+/-0.01 m m and 0.05+/-0.01 m m, respectively, with V(max)values of 120.4+/-0.5 nmol/mg/min and 41. 7+/-0.2 nmol/mg/min. Non-saturable uptake components were 29.0+/-0.1 nmol/mg/m m and 37.9+/-0.1 nmol/mg/min/m m respectively. l -leu uptake was found to be sodium-independent. The uptake of l -[(3)H]phe was strongly inhibited (90-100 per cent) by unlabelled l -phe, d -phe, l -leu or 2-aminoendobicyclo-[2,2, 1]-heptane-2-carboxylic acid (BCH) but not by l -arginine (l -arg) or methyl alpha-aminoisobutric acid (Me-AIB). Pre-incubation of Bewo cultures for 24 h in the presence of an additional 1.2 m ml -phe (simulating maternal phenylketonuria) significantly reduced both the K(m)and V(max)components of l -phe influx. l -arg (2 m m) had no effect on l -leu influx whereas 2 m ml -phe completely inhibited saturable l -leu influx. These data suggest that the microvillous border of differentiated BeWo cells transport large neutral amino acids predominantly via system L rather than by B(0) or y(+)L transporters.
    [Abstract] [Full Text] [Related] [New Search]