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  • Title: Functional and physiological consequences of genetic variation at phosphoglucose isomerase: heat shock protein expression is related to enzyme genotype in a montane beetle.
    Author: Dahlhoff EP, Rank NE.
    Journal: Proc Natl Acad Sci U S A; 2000 Aug 29; 97(18):10056-61. PubMed ID: 10944188.
    Abstract:
    Allele frequency variation at the phosphoglucose isomerase (PGI) locus in Californian populations of the beetle Chrysomela aeneicollis suggests that PGI may be undergoing natural selection. We quantified (i) apparent Michaelis-Menten constant (K(m)) of fructose 6-phosphate at different temperatures and (ii) thermal stability for three common PGI genotypes (1-1, 1-4, and 4-4). We also measured air temperature (T(a)) and beetle body temperature (T(b)) in three montane drainages in the Sierra Nevada, California. Finally, we measured 70-kDa heat shock protein (Hsp70) expression in field-collected and laboratory-acclimated beetles. We found that PGI allele 1 predominated in the northernmost drainage, Rock Creek (RC), which was also significantly cooler than the southernmost drainage, Big Pine Creek (BPC), where PGI allele 4 predominated. Allele frequencies and air temperatures were intermediate in the middle drainage, Bishop Creek (BC). Differences among genotypes in K(m) (1-1 > 1-4 > 4-4) and thermal stability (4-4 > 1-4 > 1-1) followed a pattern consistent with temperature adaptation. In nature, T(b) was closely related to T(a). Hsp70 expression in adult beetles decreased with elevation and differed among drainages (BPC > BC > RC). After laboratory acclimation (8 days, 20 degrees C day, 4 degrees C night) and heat shock (4 h, 28-36 degrees C), Hsp70 expression was greater for RC than BPC beetles. In RC, field-collected beetles homozygous for PGI 1-1 had higher Hsp70 levels than heterozygotes or a 4-4 homozygote. These results reveal functional and physiological differences among PGI genotypes, which suggest that montane populations of this beetle are locally adapted to temperature.
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