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  • Title: Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
    Author: Shida T, Hattori H, Ise F, Sekiguchi J.
    Journal: Biosci Biotechnol Biochem; 2000 Jul; 64(7):1522-5. PubMed ID: 10945275.
    Abstract:
    N-acetylmuramoyl-L-alanine amidase CwlC of Bacillus subtilis was overproduced in Escherichia coli and purified 21-fold. The amidase hydrolyzed type A cell walls such as B. subtilis. The amidase bound slightly to the Microbacterium lacticum cell wall (type B), but did not entirely hydrolyze it. The presence of calcium or magnesium ion increased the resistance of the amidase to heat denaturation.
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