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  • Title: Affinity-labelling of Escherichia coli ribosomes by a derivative of phenylalanyl-tRNA. A critical test for the specificity.
    Author: Bauer K, Czernilofsky AP, Kuechler E.
    Journal: Biochim Biophys Acta; 1975 Jun 16; 395(2):146-51. PubMed ID: 1095073.
    Abstract:
    Under nonenzymatic binding conditions p-nitrophenoxycarbonyl-phenylalanyl-tRNA can be bound to the donor as well as the acceptor site on the Escherichia coli ribosome. Precharging ribosomes with phenylalanyl-tRNA in the presence of elongation factor Tu and GTP blocks both the donor- and the acceptor site against affinity labelling by p-nitrophenoxycarbonyl-phenylalanyl-tRNA.
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