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  • Title: Rapid transbilayer phospholipid redistribution associated with exocytotic release of neurotransmitters from cholinergic nerve terminals isolated from electric ray Narke japonica.
    Author: Lee D, Hirashima N, Kirino Y.
    Journal: Neurosci Lett; 2000 Sep 08; 291(1):21-4. PubMed ID: 10962144.
    Abstract:
    Asymmetric phospholipid distribution between the outer and inner monolayers of cholinergic synaptosomal membranes at rest and their redistribution upon depolarization-induced acetylcholine (ACh) release were investigated. Translocation of phospholipids between the monolayers was measured using fluorescence-labeled phospholipid probes, NBD-PS, NBD-PE, and NBD-PC. The percentage of probes in the inner leaflet at equilibrium in synaptosomes at rest was estimated to be 63% (NBD-PS), 36% (NBD-PE), and 31% (NBD-PC). Depolarization-induced exocytosis induced rapid redistribution of these probes. Approximately 35% of PS and PC in the inner leaflet moved to the outer leaflet, whereas only 16% of PE moved. To further elucidate the mechanism of exocytosis and membrane fusion at presynaptic nerve terminals in the future, the rapid phospholipid translocation associated with exocytosis must be taken into account.
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