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  • Title: A broad-host-range plasmid for isolating mobile genetic elements in gram-negative bacteria.
    Author: Schneider D, Faure D, Noirclerc-Savoye M, Barrière AC, Coursange E, Blot M.
    Journal: Plasmid; 2000 Sep; 44(2):201-7. PubMed ID: 10964631.
    Abstract:
    Plasmid pGBG1 was constructed to isolate mobile genetic elements in a wide variety of gram-negative bacteria. The mutation target, carried on a broad-host-range vector, allows positive selection for tetracycline resistance. In tests using several gram-negative bacteria we could detect transposition events of either insertion sequences or transposons. A new insertion sequence (IS) element was identified in Ralstonia eutropha.
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