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  • Title: dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form.
    Author: Bouché JP, Zechel K, Kornberg A.
    Journal: J Biol Chem; 1975 Aug 10; 250(15):5995-6001. PubMed ID: 1097446.
    Abstract:
    The protein responsible for the initiation of conversion of single-stranded phage G4 DNA to the duplex replicative form has been purified approximately 3000-fold and identified with Escherichia coli dnaG gene product. The protein is a rifampicin-resistant RNA polymerase of approximately 64,000 daltons. It catalyzes the incorporation of the four ribonucleoside triphosphates into an oligoribonucleotide, using as template the single-stranded DNA coated with the DNA unwinding protein of E. coli. An RNA transcript of a unique region of the chromosome can serve as a primer by covalent extension by DNA polymerase III holoenzyme to form a nearly full-length linear complementary strand. A similar role for the dnaG protein in the initiation of nascent (Okazaki) fragments in replication of the host chromosome is discussed.
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