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  • Title: [Sensitive determination of vitamin K analogues in biological samples by high-performance liquid chromatography using platinum catalyst reduction and electrochemical detection].
    Author: Wakabayashi H, Onodera K.
    Journal: Nihon Yakurigaku Zasshi; 2000 Jul; 116(1):43-8. PubMed ID: 10976452.
    Abstract:
    To investigate the physiological roles of vitamin K analogues in bone metabolism, especially in osteoporosis, we have developed a sensitive and simple analysis system for vitamin K analogues in the serum and bone. After the separation of vitamin K analogues on a reversed-phase column, the analogues were reduced once in a platinum catalyst reduction column on-line and then monitored quantitatively by electrochemical detector (EICOM ECD-300) operated in the oxidation mode (+0.6 V vs. Ag/AgCl). The detection limits at a signal-to-noise ratio of 3 were 2-10 pg for vitamin K analogues. We also investigated the extraction procedures for the vitamin K analogues from serum and bone. Quantitative recoveries from serum and bone were obtained in the range of 80-101% for vitamin K analogues. We could determine both the circulating vitamin K levels in osteoporotic patients and the vitamin K contents in trabecular and cortical bone of osteoarthritis.
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