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  • Title: Improved titers of HIV-based lentiviral vectors using the SRV-1 constitutive transport element.
    Author: Mautino MR, Keiser N, Morgan RA.
    Journal: Gene Ther; 2000 Aug; 7(16):1421-4. PubMed ID: 10981670.
    Abstract:
    The development of lentiviral vectors that use Rev-independent mechanisms of nuclear export for their genomic RNA could facilitate the construction of novel anti-HIV vectors. We have improved the titers of Rev-independent lentiviral vectors having the SRV-1 CTE by mutating the major splice donor and acceptor sites present in the vector and by relocalization of the CTE sequences adjacent to the HIV-1 3'LTR. These two modifications have additive beneficial effects on vector titers and packaging efficiency. Packaging these CTE+ vectors expressing marker genes with a Rev-dependent HIV-1 helper vector yields higher titers than are obtained using a Rev-dependent lentiviral vector.
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