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  • Title: Determination of serum nucleotidase with cytidine monophosphate as substrate, (I).
    Author: van der Kooij PJ, Persijn JP, van der Slik W, Korsten CB.
    Journal: Z Klin Chem Klin Biochem; 1975 Apr; 13(4):149-55. PubMed ID: 1098294.
    Abstract:
    This paper deals with a new method for the determination of serum nucleotidase (EC 3.1.3.5). The assay is performed with cytidine-5'-monophosphate as substrate, followed by deamination of the generated cytidine. The principle of the method and the determination of the liberated ammonia by the Berthelot indophenol-reaction are comparable to the Persijn--van der Slik method in which adenosine-5'-monophosphate is used as substrate. The correlation between the results obtained with these two methods was found to be good; the new method has the advantage of higher sensitivity.
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