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  • Title: A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus.
    Author: Eroglu C, Yildiz E, Ozturk M, Pinarbasi E.
    Journal: Acta Virol; 2000 Feb; 44(1):29-33. PubMed ID: 10989689.
    Abstract:
    In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned, sequenced, expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3rd generation enzyme immunoassay (EIA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.
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