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  • Title: Detection of small round structured viruses in stool specimens from outbreaks of gastroenteritis by electron microscopy and reverse transcription-polymerase chain reaction.
    Author: Otsu R, Ishikawa A, Mukae K.
    Journal: Acta Virol; 2000 Feb; 44(1):53-5. PubMed ID: 10989693.
    Abstract:
    In testing 60 stool specimens small round structured virus (SRSV) particles were detected in 35 (58%) specimens from all 17 outbreaks of gastroenteritis by electron microscopy (EM), while SRSV genes were found in 36 (60%) specimens from 15 outbreaks by reverse transcription-polymerase chain reaction (RT-PCR) by use of 2 primer pairs. Specimens from 2 outbreaks were found SRSV particles-positive by EM but SRSV genes-negative by RT-PCR. EM remains the basic examination method for diagnosis of SRSV agents.
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