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  • Title: Amino acid sequence of the protease inhibitor BWI-4a from buckwheat seeds.
    Author: Belozersky MA, Dunaevsky YE, Musolyamov AK, Egorov TA.
    Journal: IUBMB Life; 2000 Apr; 49(4):273-6. PubMed ID: 10995028.
    Abstract:
    The complete amino acid sequence of protease inhibitor BWI-4a from buckwheat (Fagopyrum esculentum Moench) seeds, consisting of 67 amino acid residues with a single disulfide bond, has been established by Edman degradation in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Its N terminus is blocked by a pyroglutamic acid residue. Mass spectrometric analysis revealed that inhibitor BWI-4a is present in buckwheat seeds in two isoforms with a single amino acid substitution of Ala40 for Gly40. The reactive site of the inhibitor contains an Arg43-Asp44 bond. Analysis of the amino acid sequence suggests that the buckwheat seed protease inhibitor is a member of the potato proteinase inhibitor I family.
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