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  • Title: Effect of CDP-choline on brain acetylcholinesterase and Na(+), K(+)-ATPase in adult rats.
    Author: Plataras C, Tsakiris S, Angelogianni P.
    Journal: Clin Biochem; 2000 Jul; 33(5):351-7. PubMed ID: 11018686.
    Abstract:
    OBJECTIVE: The aim of this work was to investigate the effect of different cytidine-5'-diphosphocholine (CDP-choline) concentrations (0.1-1 mM) on acetylcholinesterase (AChE), (Na(+),K(+))-ATPase and Mg(2+)-ATPase activities in homogenates of adult rat whole brain and in pure (nonmembrane bound) enzymes. DESIGN AND METHODS: Tissues were homogenized, centrifuged at 1000 xg for 10 min, and in the supernatant AChE activity and Na(+),K(+)-ATPase, Mg(2+)-ATPase activities were determined according to Ellman's method and Bowler's and Tirri's, respectively. RESULTS: After a 1-3 h CDP-choline preincubation, this substance induced a maximal stimulation of 20%-25% (p < 0.001) for AChE and 50-55% (p < 0.001) for Na(+), K(+)-ATPase, but it did not influence Mg(2+)-ATPase activity. One mM acetylcholine (ACh) resulted in an approximately 18% (p < 0.001) AChE inhibition by excess substrate in the brain homogenate, while 0. 01 mM noradrenaline did not influence Na(+),K(+)-ATPase activity. CONCLUSIONS: CDP-choline can stimulate brain AChE and Na(+), K(+)-ATPase independently of ACh and noradrenaline. This enzymatic stimulation may be due to the transformation of CDP-choline to membrane phophatidylcholine. The above data could explain in part the clinical effects of this substance in some neuronal disturbances.
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