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  • Title: Lipoxygenase metabolism following laser induced retinal injury in rabbits.
    Author: Naveh N, Weissman C, Marshall J.
    Journal: Curr Eye Res; 2000 Jul; 21(1):554-9. PubMed ID: 11035536.
    Abstract:
    PURPOSE: 1) to investigate whether leukotriene B(4) (LTB(4)) is a factor in the inflammatory reaction following chorioretinal laser injury in rabbits; 2) to study its relationship with the cyclooxygenase (COX) metabolic pathway; 3) to study the influence of Nordihydroguaiaretic acid (NDGA), an inhibitor; of the lipoxygenase (LOX) cascade, on both COX and LOX metabolism. METHODS: Prostaglandin E(2) (PGE(2)) and LTB(4) synthesis by incubated samples of chorioretina obtained from rabbits' eyes exposed to Neodymium:Yag laser along with these eicosanoids accumulation in the vitreous were measured over one week follow-up period. The effect of NDGA pre-treatment on the COX and the LOX pathways in the laser-injured chorioretina was also assessed. PGE(2) and LTB(4) levels in the vitreous and in the chorioretina incubation medium were quantified using the radioimmunoassay technique with the appropriate antibodies. RESULTS: LTB(4) in vitro production by rabbits' chorioretina subjected to ND; YAG laser was significantly elevated compared to control, peaking on day 7 to levels 2.45 fold greater than baseline (p < 0.01). PGE(2) formation, following a different pattern, was also enhanced and its maximal level (5.2 fold higher than control, p < 0.01) was achieved at the initial phase (day 1 post laser). Laser irradiation caused also an increase in the two eicosanoids accumulation in the vitreous, which was however not proportional to their production levels. NDGA treatment was associated with a sustained decrease in LTB(4) content in the vitreous, but had no effect on PGE(2) vitreal levels. CONCLUSIONS: Laser irradiation of the rabbits' retina induces an alteration in the LOX metabolic pathway, which is dissociated from the influence on the COX cascade, pointing for the first time to a possible role played by LTB( 4) as a mediator in the chorioretinal inflammatory reaction, with no connection to the role played by PGE(2). NDGA selectively inhibited LOX activity without affecting COX activity.
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