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  • Title: [Content determination of baicalin and baicalein in shuanghuanglian powdery injection by the method of HPLC-ECD].
    Author: He X, Shi C, Feng Y, Kohri N, Iseki K, Miyazaki S.
    Journal: Zhongguo Zhong Yao Za Zhi; 1997 Oct; 22(10):604-5, 639-40. PubMed ID: 11038928.
    Abstract:
    The contents of baicalin and baicalein in Shuanghuanglian Powdery Injection have been determined by the method of HPLC-ECD under following conditions: column-ERC-ODS-1161, 6 mm x 100 mm; temperature-55 degrees C; mobile phase-0.1 mol/L KH2PO4, acetic acid and tetrahydrofuran (1000:100:190); flow rate-0.8 ml/min; detector voltage-+650 mV. The result shows the limit of detection for baicalin and baicalein to be 25 ng and 50 ng respectively, with an average recovery of 100.1% and 99.7% respectively. Highly reproducible, rapid, simple, convenient and accurate, this method is good for the content determination of baicalin and baicalein in Chinese medicinal herbs and preparations.
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