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  • Title: Cross-linking studies on the 50 S ribosomal subunit of Escherichia coli with methyl 4-mercaptobutyrimidate.
    Author: Kenny JW, Sommer A, Traut RR.
    Journal: J Biol Chem; 1975 Dec 25; 250(24):9434-6. PubMed ID: 1104622.
    Abstract:
    The 50 S ribosomal subunits of Escherichia coli were incubated with methyl 4-mercaptobutyrimidate and the formation of intermolecular protein:protein disulfide bonds was promoted by oxidation. Cross-linked proteins were analyzed by diagonal polyacrylamide/sodium dodecyl sulfate gel electrophoresis. Three pairs of cross-linked proteins were identified: L2-L7,L12; L5-L7,L12; and L17-L32. The significance of the results in relation to the location of sites for factor binding, peptidyltransferase, and GTP hydrolysis is discussed.
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