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  • Title: [Measurement of reticulated platelet using whole blood: methodology and clinical application].
    Author: Nishiyama M, Hayashi S, Futsukaichi Y, Suehisa E, Kurata Y.
    Journal: Rinsho Byori; 2000 Sep; 48(9):872-6. PubMed ID: 11051807.
    Abstract:
    We established simple and rapid method to measure reticulated platelets using whole blood. After whole blood was fixed with paraformaldehyde, sample was stained with thiazole orange and analyzed using flow cytometer. %RP was significantly higher in patients with idiopathic thrombocytopenic purpura(ITP) (27.7 +/- 14.3%) compared with that of normal subjects(11.9 +/- 3.1%), and elevated in 73% of ITP patients. Furthermore, %RP reflected the status of disease very well in a patient with ITP undergoing therapy. Our method can be effectively applied for differential diagnosis and assessment of the status of thrombocytopenic disorders.
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