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Title: Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli. Author: Miller S, Ness LS, Wood CM, Fox BC, Booth IR. Journal: J Bacteriol; 2000 Nov; 182(22):6536-40. PubMed ID: 11053405. Abstract: A new subunit, YabF, for the KefC K(+) efflux system in Escherichia coli has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.[Abstract] [Full Text] [Related] [New Search]