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  • Title: Cloning, expression, and characterization of pyrrolidone carboxyl peptidase from the archaeon Thermococcus litoralis.
    Author: Singleton MR, Taylor SJ, Parrat JS, Littlechild JA.
    Journal: Extremophiles; 2000 Oct; 4(5):297-303. PubMed ID: 11057915.
    Abstract:
    The gene encoding pyrrolidone carboxyl peptidase (Pcp) has been cloned from the hyperthermophilic archaeon Thermococcus litoralis. The recombinant enzyme has been expressed in Escherichia coli, purified, and characterized. The T. litoralis Pcp demonstrates strong sequence homology to previously characterized bacterial Pcps. Some investigations have been carried out on enzyme substrate specificity and stability.
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