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  • Title: [Demonstration by direct immunofluorescence of intracellular immunoglobulins on sections of tissues fixed in Bouin's fluid and embedded in paraplast].
    Author: Diebold J, Tricot G, Berault J, Camilleri JP.
    Journal: Ann Anat Pathol (Paris); 1975; 20(2):183-90. PubMed ID: 1106264.
    Abstract:
    Sections obtained from lymphoid tissues fixed immediately after biopsy in Bouin's fluid and embedded in paraplast may be used to visualize the immunosecretory cells with classical direct immunofluorescence. A comparative study of the same fragments processed according to the frozen section method followed by acetone fixation or according to Sainte-Marie's method confirms the value and the advantages of the proposed technique. Early fixation of thin fragments seems to be the main point.
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