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  • Title: Determination of T-2 toxin in grain and grain products by HPLC and TLC.
    Author: Omurtag GZ, Yazicioğlu D.
    Journal: J Environ Sci Health B; 2000 Nov; 35(6):797-807. PubMed ID: 11069019.
    Abstract:
    The purpose of this study was to investigate the T-2 toxin contaminated grain and grain products consumed especially by Turkish population. The T-2 toxin was detected using the high performance liquid chromatography (HPLC) with UV detector at 208 nm and the identify of T-2 was further confirmed by thin layer chromatography (TLC). The recovery was 91 +/- 4.24% for corn flour fortified with the known amount of T-2 toxin (1 ppm). The detection limits of T-2 toxin for the HPLC and the TLC were 25 ng and 50 ng, respectively. A total of 30 commercially available grain and grain product samples were analyzed. Two corn flour samples were found to contain detectable levels of T-2 toxin at a level of 1.60 ppm and 4.08 ppm.
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