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  • Title: Suppression of eukaryotic translation termination by selected RNAs.
    Author: Carnes J, Frolova L, Zinnen S, Drugeon G, Phillippe M, Justesen J, Haenni AL, Leinwand L, Kisselev LL, Yarus M.
    Journal: RNA; 2000 Oct; 6(10):1468-79. PubMed ID: 11073222.
    Abstract:
    Using selection-amplification, we have isolated RNAs with affinity for translation termination factors eRF1 and eRF1.eRF3 complex. Individual RNAs not only bind, but inhibit eRF1-mediated release of a model nascent chain from eukaryotic ribosomes. There is also significant but weaker inhibition of eRF1-stimulated eRF3 GTPase and eRF3 stimulation of eRF1 release activity. These latter selected RNAs therefore hinder eRF1.eRF3 interactions. Finally, four RNA inhibitors of release suppress a UAG stop codon in mammalian extracts dependent for termination on eRF1 from several metazoan species. These RNAs are therefore new specific inhibitors for the analysis of eukaryotic termination, and potentially a new class of omnipotent termination suppressors with possible therapeutic significance.
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