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  • Title: Genotyping of clinical and chicken isolates of Campylobacter jejuni and Campylobacter coli.
    Author: Smith SI, Olukoya DK, Fox AJ, Coker AO.
    Journal: Cytobios; 2000; 103(403):91-101. PubMed ID: 11077971.
    Abstract:
    Genomic DNA from 58 strains of Campylobacter made up of 48 Campylobacter jejuni and ten Campylobacter coli were digested with Sma I and analysed by pulsed-field gel electrophoresis (PFGE). The cleavage of DNA by Sma I gave 22 distinct hybridization patterns. The two Campylobacter species were subtyped by PFGE. The average genomic size for C. jejuni by Sma I digestion was 1.73 Mb, while that of C. coli gave 1.7 Mb. Results from this study indicate that PFGE analysis by Sma I digested genomic DNA provides a reliable means of differentiating between and within species of Campylobacter and provides a practical approach to epidemiological studies of Campylobacter.
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