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  • Title: Inhibition of eukaryotic ribonuclease P activity by aminoglycosides: kinetic studies.
    Author: Tekos A, Tsagla A, Stathopoulos C, Drainas D.
    Journal: FEBS Lett; 2000 Nov 17; 485(1):71-5. PubMed ID: 11086168.
    Abstract:
    The effect of several aminoglycoside antibiotics on ribonuclease P (RNase P) was investigated using an in vitro experimental system from Dictyostelium discoideum. Detailed kinetic analysis showed that all aminoglycosides tested (tobramycin, gentamicin, kanamycin, paromomycin, neomycin) behave as classical non-competitive inhibitors, with neomycin being the strongest inhibitor. The inhibition effect is attributed to the electrostatic competition of the cationic aminoglycosides with magnesium ions required for catalysis. Increasing Mg(2+) ion concentrations reduced the effect of aminoglycosides on RNase P activity. Detailed kinetic analysis showed that aminoglycosides compete with Mg(2+) for common binding sites on RNase P holoenzyme.
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