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  • Title: Europium cryptate labeled deoxyuridine-triphosphate analog: synthesis and enzymatic incorporation.
    Author: Alpha-Bazin B, Bazin H, Guillemer S, Sauvaigo S, Mathis G.
    Journal: Nucleosides Nucleotides Nucleic Acids; 2000 Sep; 19(9):1463-74. PubMed ID: 11092315.
    Abstract:
    The synthesis of an europium tris-bipyridine cryptate labeled 2'-deoxyuridine-5 '-triphosphate analog (K-11-dUTP) is described. This labeled triphosphate was incorporated into DNA through enzymatic reactions with terminal transferase and DNA polymerases. The enzymatic reactions were monitored by TRACE (Time Resolved Amplification of Cryptate Emission), a homogeneous method using Fluorescence Resonance Energy Transfer (FRET) from an europium cryptate as donor to a modified allophycocyanine as acceptor.
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