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  • Title: Production and purification of recombinant human alpha 2C2 adrenergic receptor using Saccharomyces cerevisiae.
    Author: Kapat A, Jaakola VP, Heimo H, Liitti S, Heikinheimo P, Glumoff T, Goldman A.
    Journal: Bioseparation; 2000; 9(3):167-72. PubMed ID: 11105246.
    Abstract:
    The objective is to generate milligram quantities of recombinant human alpha 2C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.
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