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  • Title: Opening of the active site of Clostridium perfringens alpha-toxin may be triggered by membrane binding.
    Author: Titball RW, Naylor CE, Miller J, Moss DS, Basak AK.
    Journal: Int J Med Microbiol; 2000 Oct; 290(4-5):357-61. PubMed ID: 11111911.
    Abstract:
    On the basis of amino acid sequence homologies with other phospholipases C, the alpha-toxin of Clostridium perfringens was predicted to be a two-domain protein. Using truncated forms of alpha-toxin the phospholipase C active site was shown to be located in the amino-terminal domain. Crystallographic studies have confirmed this organisation and have also revealed that the carboxy-terminal domain is structurally similar to the phospholipid-binding domains in eukaryotic proteins. This information has been used to devise a model predicting how alpha-toxin interacts with membranes via calcium-mediated recognition of phospholipid head groups and the interaction of hydrophobic amino acids with the phospholipid tail group. The binding of alpha-toxin to membranes appears to result in the opening of the active site allowing hydrolysis of membrane phospholipids.
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