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  • Title: Separation and determination of alditols and sugars by high-pH anion-exchange chromatography with pulsed amperometric detection.
    Author: Andersen R, Sørensen A.
    Journal: J Chromatogr A; 2000 Nov 03; 897(1-2):195-204. PubMed ID: 11128203.
    Abstract:
    Carbohydrates such as alditols (polyols or sugar alcohols), monosaccharides and disaccharides are separated as anions by anion-exchange chromatography with a sodium hydroxide eluent, MA1 CarboPac column and pulsed amperometric detection. We report a high-pH anion-exchange chromatographic-pulsed amperometric detection (HPAEC-PAD) method that determines all the polyols used as food additives in food products and the most commonly found mono- and disaccharides on a routine basis. The linearity, repeatability, internal reproducibility and accuracy are described. The applicability of the method has been demonstrated by the analysis of 46 relevant samples and by participation twice in the Food Analysis Performance Assessment Scheme (FAPAS) testing programme for food additives.
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