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  • Title: Molecular cloning and expression of the nucleoside deoxyribosyltransferase-II gene from Lactobacillus helveticus.
    Author: Okuyama K, Noguchi T.
    Journal: Biosci Biotechnol Biochem; 2000 Oct; 64(10):2243-5. PubMed ID: 11129605.
    Abstract:
    Nucleoside deoxyribosyltransferase-II, which catalyzes transfer of glycosyl residues from a donor deoxynucleoside to an acceptor base, was purified from Lactobacillus helveticus and its gene was cloned. Analysis of the nucleotide sequence showed the presence of a 474-nucleotide open reading frame encoding a protein of 158 amino acids with a molecular weight of 18,317. The active enzyme can be produced in large quantities in E. coli cells using the cloned gene.
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