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  • Title: A rapid method for the measurement of estradiol and hydrocortisone levels in maternal and fetal blood and amniotic fluid.
    Author: Sybulski S, Maughan GB.
    Journal: Am J Obstet Gynecol; 1975 Jan 01; 121(1):32-6. PubMed ID: 1115113.
    Abstract:
    Estradiol and hydrocortisone levels in maternal and fetal (umbilical cord) blood in human pregnancy can be simultaneously assessed by this method. Chromatography on microcolumns of Sephadex LH-20 with benzene:methanol (85:15) as developing solvent is used to separate estradiol, hydrocortisone, and isatin (a yellow-colored substance used as marker) from interfering steroids. Human late pregnancy plasma is used as the source of both sex hormone-binding globulin and transcortin for the competitive protein-binding assay of estradiol and hydrocortisone, respectively. The method is rapid enough to permit the monitoring of a high-risk pregnancy for plasma estradiol levels on a daily basis. The method is sensitive enough to permit assessment of perinatal adrenocortical function. Hydrocortisone levels can be measured in as little as 3 ml. of amniotic fluid obtained by amniocentesis and preliminary results indicated that hydrocortisone can be determined in as little as 0.1 ml. of fetal scalp blood or newborn baby blood.
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