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  • Title: Characterization of the genome of Phycomyces blakesleeanus.
    Author: Dusenbery RL.
    Journal: Biochim Biophys Acta; 1975 Feb 10; 378(3):363-77. PubMed ID: 1115786.
    Abstract:
    DNA of Phycomyces blakesleeanus was extracted from whole mycelia and from nuclear and mitochondrial organelle fractions obtained from sporangiophores. DNA from all three sources exhibits one symmetrical band at p equals 1.688 g/ml in CsCl buoyant density gradients. Reassociation data are consistent with kinetic division of the DNA into three components: very rapidly renaturing (fraction I), rapidly reassociating (fraction II) and slowly reassociating (fraction III) base sequences. These components comprise approximately 10%, 20% and 64% of total cell DNA. Kinetic fractions were prepared from total cell DNA and reassociated separately. The corrected rate constant is 11.3 M-1-S-1 for the rapidly reassociating component and 0.055 M-1-S-1 for the slowly reassociating component. Based on these data and the data from unfractionated total cell DNA, the genome size of Phycomyces is approximately 1.9-10-10 daltons, 6.7 times that of Escherichia coli.
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