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  • Title: Biochemical characterization of an active pyrophosphate-dependent phosphofructokinase from Treponema pallidum.
    Author: Roberson RS, Ronimus RS, Gephard S, Morgan HW.
    Journal: FEMS Microbiol Lett; 2001 Jan 15; 194(2):257-60. PubMed ID: 11164318.
    Abstract:
    An active pyrophosphate-dependent phosphofructokinase containing a six residue polyhistidine tag has been cloned from Treponema pallidum, and characterized biochemically. The phosphofructokinase has pH optima for activity of 8.0 for both the forward and reverse reactions. The apparent K(m) for pyrophosphate was 0.042 mM (V(max) of 141 U mg(-1) protein) and for fructose-6-phosphate, 0.529 mM. The apparent K(m) for the reverse reaction for fructose-1,6-diphosphate was 0.267 mM (V(max) of 42.4 U mg(-1) protein). The enzyme appears to be both a dimer and non-allosteric.
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