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  • Title: Effect of sperm numbers on fertility of frozen bull spermatozoa in skim milk diluent.
    Author: Almquist JO.
    Journal: J Dairy Sci; 1975 Mar; 58(3):420-2. PubMed ID: 1117080.
    Abstract:
    Insemination doses of 10, 15, and 20 million progressively motile spermatozoa prior to freezing were compared in a split-ejaculate fertility experiment involving 4,603 first service inseminations to 34 semen samples from six Angus bulls. Based on 60- to 90-day nonreturn rates, fertility was significantly lower for inseminating doses of 10 and 15 times 10-6 than 20 times 10-6 motile sperm (61, 62, and 70%). The 1-ml doses of skim milk diluted semen were frozen in glass ampules. The post-thaw number of motile sperm per ampule after freezing and storage for 3 wk in liquid nitrogen averaged 5.5, 8.4, and 12.4 times 10-6 for corresponding pre-freeze numbers of 10, 15 and 20 times 10-6 motile sperm/ml and ranged from 4.0 to 15.7 times 10-6/ml. Frozen semen packaged in ampules should contain more than 8.4 times 10-6 motile cells post-thaw per ampule.
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