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  • Title: Quantitative extraction of tubocurarine, gallamine, and decamethonium from biological materials.
    Author: Wittmer D, Atwell S, Haney WG.
    Journal: J Forensic Sci; 1975 Jan; 20(1):86-90. PubMed ID: 1117276.
    Abstract:
    A procedure is described for quantitative extraction of tubocurarine, gallamine, and decamethonium from blood, urine, and tissue. Ion pairs of the drugs are formed with picric acid and are extracted into a dichloromethane/1-pentanol solvent. Qualitative analysis of the extract is effected using thin-layer chromatography. Quantitative analysis is accomplished by elution of TLC areas containing the drug, formation of the ion pair with sodium 9,10-dimethoxyanthracene-2-sulfonate, and analysis of the ion pair by spectrophotofluorometry.
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