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  • Title: Atomic force microscopy of RecA--DNA complexes using a carbon nanotube tip.
    Author: Umemura K, Komatsu J, Uchihashi T, Choi N, Ikawa S, Nishinaka T, Shibata T, Nakayama Y, Katsura S, Mizuno A, Tokumoto H, Ishikawa M, Kuroda R.
    Journal: Biochem Biophys Res Commun; 2001 Feb 23; 281(2):390-5. PubMed ID: 11181060.
    Abstract:
    We report high resolution images of RecA-double stranded (ds) DNA complexes obtained by atomic force microscopy (AFM). When a carbon nanotube (CNT) tip was used, AFM images visualized the 10-nm pitch of RecA-dsDNA complexes and RecA filaments as three-dimensional surface topography without reconstruction analysis. The depth of the notch between two pitches was less than 1 nm. When adsorbed on a soft surface covered with proteins, naked DNA, RecA monomers, RecA hexamers, and short RecA filaments were all clearly resolved in one image. The high resolution images with a CNT tip provided valuable information on the initiation process of RecA-dsDNA complex formation.
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