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  • Title: [A comparative study of serological, monoclonal antibody and DNA typing in identifying HLA-A].
    Author: Tan J, Tang X, Ding Y.
    Journal: Zhonghua Xue Ye Xue Za Zhi; 1998 Aug; 19(8):395-7. PubMed ID: 11189503.
    Abstract:
    OBJECTIVE: A double-blind study was carried out to evaluate the accuracy and reliability of PCR-SSP assay in comparison with serology and monoclonal antibody(mAb) typing in identifying HLA-A alleles in Southern Chinese population. METHODS: A total of 296 samples were entered into the study, including 143 unrelated kidney donors and 153 recipients. HLA-A typing was performed by standard two-stage microlymphocytotoxicity assay, one-step mAb typing and PCR-SSP typing. RESULTS: All samples were successfully typed by PCR-SSP. Reproducibility was 100%. The results were confirmed by a panel of standard DNAs and a double-blind typing of UCLA tissue typing lab. However, mAb typing(for Asian) in 149 samples showed 2.7% misassignment including 1 antigen being incorrectly interperted and 3 of 26 "blanks" turning out to be definable alleles by DNA typing. Serological discrepancy rate was 15.6% in 147 samples consisting of 8 antigens being incorrectly interpreted, 13 "blanks" turning out to be definable alleles and 2 heterozygotes turning out to be homozygotes by DNA typing. CONCLUSION: HLA-A typing by PCR-SSP proved to be a rapid and accurate technique, suitable for clinical application with a greater precision than serology. In large scale screening, mAb typing (for Asian) is recommended. Antigens of "blanks" or "difficult" by serology or mAb typing should be retyped by DNA typing.
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