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  • Title: Globin mRNA from rabbit reticulocyte membrane-bound ribosomes.
    Author: Brennessel BA, Goldstein J.
    Journal: Biochim Biophys Acta; 1975 Jan 06; 378(1):73-9. PubMed ID: 1120136.
    Abstract:
    Messenger RNA has been isolated from both free and membrane-bound rabbit reticulocyte ribosomes. Membrane-bound ribosomes, which constitute less than 10% of total cellular ribosomes, are released from the membrane by deoxycholate but remain associated with the membrane fraction following treatment with 0.5 M KC1. The major messenger RNA species isolated from either free or bound ribosomes is globin messenger. Both alpha- and beta-globin chains are synthesized when the isolated messenger RNA from either free or membrane-bound reticulocyte ribosomes is added to a Krebs ascites cell-free system.
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