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  • Title: Fluorescamine as a terminating agent in solid phase peptide synthesis.
    Author: Felix AM, Jimenez H, Vergona R, Cohen MR.
    Journal: Int J Pept Protein Res; 1975; 7(1):11-22. PubMed ID: 1120631.
    Abstract:
    Fluorescamine was shown to be an excellent terminating agent for blocking unreacted amino groups during solid phase peptide synthesis. A comparison of the termination efficiency of fluorescamine versus that of acetylation revealed that the former method gave superior products as assessed by peptide analysis, dansyl-amino end group determination and biological assay. In addition, fluorescamine terminated fragments were converted to non-fluorescent spirolactones during the deprotection stage. These spirolactones were stable to subsequent solid phase reaction conditions and were readily removed from the target peptide.
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