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  • Title: Long-term regulation of catecholamine formation by ouabain in cultured bovine adrenal chromaffin cells.
    Author: Ozawa Y, Houchi H, Teraoka K, Azuma M, Kamimura T, Yoshizumi M, Tsuchiya K, Tamaki T, Minakuchi K.
    Journal: J Cardiovasc Pharmacol; 2000; 36 Suppl 2():S15-8. PubMed ID: 11206714.
    Abstract:
    The long-term effects of ouabain, an inhibitor of Na+/K+ -ATPase, on catecholamine formation in cultured bovine adrenal chromaffin cells were examined. The increase in [14C]catecholamine formation from [14C]tyrosine induced by ouabain was dependent on incubation time, and its maximal effect was observed after incubation for 8 h. The stimulatory effect of ouabain was concentration dependent (10-300 nM), causing maximal stimulation at 300 nM. The formation of [14C]catecholamines induced by ouabain was not increased by incubation with [14C]DOPA instead of [14C]tyrosine. Ouabain-induced [14C]catecholamine formation was influenced by decreases in extracellular Ca2+ concentration, but not by the presence of cycloheximide or actinomycin D. These results suggested that ouabain stimulates continuous activation of hydroxylation of tyrosine through a Ca2+ -dependent mechanism in cultured bovine adrenal chromaffin cells.
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