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  • Title: Continuous separation of green fluorescent protein by annular chromatography.
    Author: Uretschläger A, Einhauer A, Jungbauer A.
    Journal: J Chromatogr A; 2001 Jan 26; 908(1-2):243-50. PubMed ID: 11218127.
    Abstract:
    The concept of annular chromatography was tested by separation of a real protein solution used in biotechnology. Green fluorescent protein was expressed in S. cerevisiae and the extract was continuously separated by a pressurized annular chromatograph packed with a Superdex 200 prep grade size-exclusion chromatography medium. Purity was checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting and fluorescent intensity. The continuous mode was compared to batchwise operation. Under the assumption that equivalent fractions are collected, both modes are comparable.
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