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  • Title: [Determination of iron with CentrifiChem System].
    Author: Eisenwiener HG.
    Journal: Z Klin Chem Klin Biochem; 1975 Jan; 13(1):21-4. PubMed ID: 1121896.
    Abstract:
    The determination of iron was adapted to the CentrifiChem System. The iron bound to transferrin is freed with a detergent, reduced to Fe++ with sodium dithionite, and determined with bathophenanthroline disulphonate. The operation consists of one run for the blank value and one analytical run. Although the actual reaction time is extremely short, a reaction time of 8--10 min is recommended for both the blank value and the analysis. This ensures adequate clearing during centrifugation under the influence of Teepol. 2 times 100 mul serum are required, and 80 determinations per hour are possible.
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